Sentence examples for buffer sections were incubated from inspiring English sources

After removal of blocking buffer, sections were incubated at 4°C overnight with fluorescein-reporter conjugated and Biotin conjugated c-Met antibodies prepared in blocking buffer.

After rinses with buffer, sections were incubated with Texas-red labeled goat anti-rabbit IgG (Molecular Probes) for 30 min.

After blotting off excess buffer, sections were incubated in primary anti-FGF-8b antibody diluted 1/3 with blocking buffer at RT for 2 h.

After washing for 10 min in DAKO buffer, sections were incubated for a further hour in rabbit/mouse EnVision (DAKO) conjugated to horseradish peroxidase.

After washing three times with TNT wash buffer, sections were incubated for 30 min at RT with an appropriate secondary antibody conjugated with horse-radish peroxidase.

After two washes with binding buffer, sections were incubated with 1 μM random sequence and then incubated with 250 nM SYL3C-Cy3 for 1 h.

After enzyme or buffer treatment, sections were incubated in phosphate-buffered saline (PBS) containing 5% (w/v) milk protein (MP/PBS) and a 5-fold dilution of antibody hybridoma supernatant for 1.5 h.

After being washed with the blocking buffer, the sections were incubated with the appropriate secondary antibody for 1 h at room temperature and extensively washed with PBS.

After washing 3 times in 0.05M Tris buffer, the sections were incubated with secondary antibodies (see Table 2) for 1 hr at RT. Nucleic acid staining was carried out by labeling with Bisbenzamide for 10 minutes.

Immunolabelling: After a blocking step of two times 10 mn in glycine (0,5 mM in PBS) and another 10 mn in 1% BSA/PBS (blocking buffer), the sections were incubated 20 min with the primary antibodiy.

Polyclonal rabbit anti-mouse fibrinogen (Dako, Carpinteria, CA) was diluted 1 200 in blocking buffer and sections were incubated overnight for 16 hours at 4°C.