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After a final wash in PBS buffer, sections were embedded in mounting medium and data were collected on a Zeiss LSM 510 confocal microscope (Zeiss, Oberkochen, Germany).
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After a final wash in 0.05 M Tris-HCl buffer pH 7.5, sections were embedded in mounting medium and collected on a Zeiss LSM 510 confocal microscope (Zeiss, Oberkochen, Germany).
Dried sections were embedded in Permount.
Stained sections were embedded in FluorSave (Calbiochem).
All sections were embedded and examined.
Afterwards, sections were embedded in glycerol jelly.
Dried sections were embedded in Mowiol (Calbiochem).
After washing (4×10 min) in Tris-SMB buffer, brains were embedded in agarose and cut into 60 µm sections.
The hearts stored in 10% buffered formalin, were embedded in paraffin, sections cut at 5 μm and stained with hematoxylin and eosin.
Tissues fixed in 10% buffered formalin were embedded in paraffin, sectioned at 4 μm and stained with hematoxylin and eosin (HE) and Masson's trichrome for light microscopic morphologic study.
For histological analysis, kidney tissues fixed with 4% (v/v) buffered paraformaldehyde were embedded in paraffin, and 4-μm-thick sections were prepared.
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