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After a second rinse with buffer, the sections were dehydrated in increasing concentrations of ethanol and propylene oxide and embedded in epoxy resin.
Tumors were removed from mice, and fixed in 10% buffered formalin and embedded in paraffin; 5-μm sections were dehydrated and stained with hematoxylin and eosin (H&E).
Briefly, after postfixation with 4% paraformaldehyde in phosphate buffered saline (0.1 M, pH 7.4) slide-mounted sections were dehydrated and delipidated.
In short, sections were dehydrated, and antigen retrieval was performed by heating the slides in citrate buffer, pH 6.
Sections were dehydrated and antigenic epitopes were exposed by heating in 10-mM citrate buffer (pH 6.0) or by incubation in 0.05% trypsin at +37°C.
The sections were dehydrated, embedded in paraffin and cut.
Then, sections were dehydrated.
The sections were dehydrated, and permanently mounted.
The sections were dehydrated and mounted.
The tissue sections were dehydrated and embedded in paraffin.
Finally, the sections were dehydrated, counterstained and mounted for observation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com