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Experiments were then conducted in NaHCO3/Na2CO3 buffer samples similar to conditions prevailing in the COx formation.
Its utility is well demonstrated with the sensitive and specific detection of adenosine as a model target in buffer samples and undiluted human urine within several minutes.
Their utility is well demonstrated with sensitive, specific detection of adenosine as a model analyte with the naked eye in buffer samples and undiluted human serum.
The LogDoct,pH7.4 value was calculated according to LogDoct,pH7.4 = 10Log(A oct / A buffer), with A buffer as the average radioactivity of five buffer samples and A oct as the average radioactivity of 5 1-octanol samples.
The device is tested with buffer samples containing clozapine and demonstrates a sensitivity of 54 μC mL cm−2 μg−1 and a limit-of-detection of 0.8 μg mL−1, a sensing performance similar to a counterpart macro-scale benchtop system.
Following addition of 400 µl of 1 X Binding Buffer, samples were processed by flow cytometry.
Background spectra of buffer samples were subtracted from the respective protein spectra.
After 3 washes in PBS/Tween buffer, samples are incubated for overnight at 4°C.
Matrix and buffer samples were supplemented with 13 internal standards for retention index correction (C8-C30 facid acid methyl esters) as previously reported [31].
After several washes with NETN buffer, samples were eluted by boiling in Laemmli sample buffer including β-ME and subjected to SDS-PAGE.
Following an overnight rinse in 0.1 M phosphate buffer, samples were dehydrated through a graded series of ethanol solutions, then propylene oxide.
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