Sentence examples for buffer run on a from inspiring English sources
Kidney cells were lysed in RIPA buffer, run on a 10% SDS-polyacrylamide electrophoresis gel and transferred onto a nitrocellulose membrane (Hybond C Extra, Amershan Biosciences, Little Chalfon, USA).
Aortic tissue homogenates were dissolved in sample buffer, run on a NuPAGE Novex 4 12% Bis-Tris Gel (Invitrogen, CA, United States), and transferred to nitrocellulose membranes using the iBlot transfer system (Invitrogen).
Equal amounts of proteins (30 μg) were boiled in 6× SDS PAGE loading buffer run on a polyacrylamide gel and transferred to a PVDF membrane and probed with respective antibodies.
About 20 μg of protein was loaded with SDS sample buffer, run on a 10% SDS PAGE, transferred, and probed for phospho-CaMKII at threonine 287 and GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-phospholamban (PLN) at threonine 16, phospho-PLN at threonine 17, phospho-RyR2 at serine 2808, and phospho-RyR2 at serine 2814 (Badrilla, Leeds, UK).
For immunoblotting, cells were lysed in RIPA buffer, run on an 8% gel, and transferred onto nitrocellulose.
An aliquot (10 μl, corresponding to 200 μg of total protein) of the cytochrome c-induced reaction was boiled in Laemmli buffer, run on 12% SDS PAGE, and proteins were transferred onto a nitrocellulose membrane.
Pelleted bacteria was resuspended in Laemmli sample buffer, run on SDS-PAGE and immunoblotted with each monoclonal antibody of interest.
A portion of proteins (10 µg) were denatured by boiling in Laemmli sample buffer, run on 10% SDS-polyacrylamide gels, and transferred to Immobilon-P PVDF membranes (0.45 µm pore size, Millipore).
Samples were then washed four times with lysis buffer, boiled in SDS loading buffer, run on SDS-PAGE gel and protein was detected by anti-phosphorylated or total MEKK1 antibody.
Cells were lysed in Laemmli buffer, run on SDS-PAGE and transferred on PVDF membranes.
Unless otherwise stated, wing discs or S2R+ cells were boiled in 1x or 2x SDS Sample buffer, run on 7.5% Mini-Protean TGX gels (Bio-Rad, Hercules, CA), and transferred to nitrocellulose membrane.
