Sentence examples for buffer restrictions from inspiring English sources

It further fully neglects multiple path routing as well as buffer restrictions.

Constraint sets (1) and (2) are to assure that no-buffer restrictions are held.

These modules allow making GFP constructs with or without a signal peptide and/or a His tag Restriction-ligations were performed using the optimal conditions defined previously (Promega ligation buffer, and restriction-ligation followed by heat inactivation).

The Obama administration has pledged to restore the old buffer zone restriction.

A multiple restriction digest (VspI, HindIII and HinfI) of 20 μL PCR product was performed in a final volume of 30 μL 1x restriction buffer, containing 1U of each restriction enzyme.

The fusion product was purified from agarose gel using QIAquick Gel Purification Kit (QIAGEN Inc ., resuspended in 50-μl of 1X SacI restriction buffer containing 5 U of SacI restriction enzyme, and digested for two hours at 37°C.

A 6-μl mixture was prepared consisting of 3.5 μl sterile dH2O, 2 μl of restriction enzyme buffer, and 0.5 μl restriction enzymes (HaeIII and MseI).

The plugs were then equilibrated in an appropriate restriction buffer before digesting with 500-1000 units of restriction enzyme for approximately 16 hr.

Forested buffer strips with restrictions on timber harvest and road-building (i.e. riparian buffer zones) were originally implemented to protect aquatic functions and resources.

Among the restriction enzymes, some combinations of enzymes can be used for the digestion of multiple restriction sites at once in the same reaction buffer; for examples restriction enzymes SacI and KpnI can be jointly used in the low salt buffer, EcoR I, Hind III and TaqI can be jointly used in the medium salt buffer, and BamHI, ClaI and PstI can be jointly used in the high salt buffer.

About 200 ng of genomic DNA from each sample was double digested separately with 5 U of the restriction enzymes SacI and MseI (Fermentas) with 1 × restriction buffer in a reaction volume of 25 μl.