Sentence examples for buffer resolved on a from inspiring English sources

Samples were heated at 95°C for 5 minutes after addition of 10 µL of 6 X SDS PAGE buffer, resolved on a 8% polyacrylamide gel and visualized by Coomassie staining.

An aliquot of the lysate (25 μg protein) was boiled in SDS sample buffer, resolved on a 4 12% SDS-PAGE gradient gel, and transferred to a 0.2 μm nitrocellulose membrane.

Plant protein extracts were denatured at 100°C for 10 min in SDS-PAGE gel loading buffer, resolved on a 12% acrylamide gel and transferred onto nitrocellulose membrane by semi-dry electroblotting.

Supernatants and cell lysates were boiled in a reducing NuPAGE LDS sample buffer, resolved on NuPAGE Novex 4 12 % Bis Tris Gels and electrotransferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, Norway).

Reactions were quenched at 1, 3, 10 and 30 minutes by the addition of an equal volume of 2X SDS sample buffer, resolved on 15% SDS-PAGE gels, dried, and exposed to film.

For immunoblotting, samples were boiled in an equal volume of 2 × Laemmli buffer, resolved on 7.5 or 10% SDS polyacrylamide gels and transferred to nitrocellulose membrane.

The bead fractions were resuspended in SDS sample buffer, resolved on 4 20% Tris-glycine SDS-polyacrylamide gels, and analyzed by both phosphorimaging, as well as transfer and anti-FLAG immunoblotting (see below).

Proteins were denaturated in sample buffer, resolved on SDS-PAGE gel and transferred to nitrocellulose membranes according to standard protocols.

The reactions were stopped with Laemmli sample buffer, resolved on 15% Tris-Tricine SDS-PAGE and subjected to autoradiography and quantification with Phosphor Imager (Packard Instruments, Wellesley, MA).

Then the reactions were stopped with SDS-loading buffer, resolved on 10% SDS-PAGE (Novex, Invitrogen) and subjected to autoradiography and quantification with PhosphorImager Storm 860 (Molecular Dynamics).

Beads were washed five times, eluted in boiling Laemmli buffer, resolved on 10% SDS-PAGE and immunoblotting was performed with mouse anti-alpha tubulin antibody (1∶1000).