Suggestions(1)
Exact(7)
As predicted, resistance was overestimated on maps with artificial boundaries, and the addition of a buffer removed the bias.
Incubation with thrombin during overnight dialysis at 4°C against wash buffer removed the His-tag.
After elution, samples were concentrated and elution buffer removed using ultrafiltration plates (Omega 10K membrane).
Since the proteins contained poly-histidine tags, they efficiently bound the copper chelate while a subsequent rinse with fresh buffer removed excess proteins.
Following this, the column was washed four times with MACS buffer, removed from the magnet and the cells eluted with 2 ml MACS buffer.
Intact, unfixed islets were then transferred to microscope slides, excess buffer removed using a stretched Pasteur pipette, and a cover slip sealed over the islets.
Similar(53)
Defining the input as circular buffer removes the overhead of an additional branch instruction inside the loop.
Rinsing with PBS buffer removes the weakly bound fraction and leaves a single molecular layer at 1.1 nm thickness, along with concentration dependent surface coverage.
The conjugate was purified with a spin filter (Amicon, Millipore, USA) using 0.9% NaCl as running buffer removing daughter nuclides and non-chelated 227Th.
This stripping buffer removes antibodies from membranes very effectively.
Buffers removed an average of 70% of P and 78% of N to reduce nutrients entering the playa.
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