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The buffer range spanned from pH 3.3 to 5.3 with optimal buffering at pH 4.35 and a buffer value of 0.004 mol/(l×pH).
The buffer range spanned from pH 5 to 7 with optimal buffering at pH 6.3 and a buffer value of 0.0005 mol/(l×pH) and from 7.2 to 9 with optimal buffering at pH 8.4 and a buffer value of 0.0006 mol/(l×pH).
The buffer range spanned from pH 3.3 to 5.3 with an optimal buffering at pH 4.5 and a buffer value of 0.007 mol/(l×pH).
The protein was then eluted with a gradient of imidazole in wash buffer (range 50 250 mM) with detection at 280 nm.
The size pH curve was characterized by a smooth sigmoid form, and had a dramatic upward shift with decreasing pH from 6.5 to 4.5, which correlated well with the buffer range of hydrophilic PADMC.
The buffer range is shifted into the mild alkaline range, and both, increased temperature and increased pH value, cause transition of ammonium to the toxic-free ammonia.
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Amyloglucosidase and lysozyme have the ideal physicochemical properties to demonstrate buffering beyond the buffer ranges of di-hydrogenphosphate and hydrogencarbonate.
Less than 2% of the adsorbed Cry1Ab proteins were desorbed using H2OMQ, while a considerable proportion of the adsorbed proteins could be desorbed with the buffer, ranging from 20%to40%0%.
In the current study, we have developed an enzyme-linked immunosorbent assay (ELISA -based protELISA -based microarray for the sensitive and simultaneous detection of BoNT serotyproteinB, C, D, E, antibodyith engineered high-affinity antibodies, the BoNT assays have sensitivities in buffer ranging fromicroarray0.2 pg/ml) to 14.7 for(2.2 pg/ml).
Human whole saliva had a buffer zone spanning from pH 3.4 to 8 compassing the buffer ranges of hydrogencarbonate (pH 5.1 to 7.1) and di-hydrogenphosphate (pH 6.1 to 8.1).
100 µl of sample dilutions (plasma dilutions in EIA buffer, ranging from 1∶100 to 1∶1000 for mTA12 and from 1∶5 to 1∶20 for cTA12, or purified antibody for calibration) was added to the wells with 50 µl of 2 EU/ml competitor (mTA12 tracer antibody) and 50 µl of 0.1 nM Fc-BoNT/A1.
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