Sentence examples for buffer provided with the from inspiring English sources

All wells were washed three times with diluted (1 ×) wash buffer provided with the kit.

For preparation of miRNA containing total RNAs, ~100 mg of liver tissue was homogenised in lyses buffer provided with the mirVana™ miRNA Isolation Kit (P/N: 1560, Ambion, Austin, TX) and total RNA were prepared according to the manufacturer's protocol.

The GBM tumor sample (∼100 150 mg) was homogenized in 1 ml lysis buffer provided with the RNAqueous-4PCR kit.

HIV-1 genomic RNA was extracted (Qiagen UltraSens RNA kit) from 200 µL plasma or serum and reconstituted in 50 µL of buffer provided with the kit.

In vitro transcribed, gel purified and refolded RNA (1 µg) was mixed with yeast RNA (1 µg) and structure buffer provided with the RNases (Ambion).

The cells were lysed in the luciferase cell culture lysis buffer provided with the Luciferase Assay Kit (Promega, Madison, USA) and 15 µl of supernatant was analyzed for firefly luciferase activity.

Slide hybridization and washing steps were performed according to manufacture's protocol using buffers provided with the kit (Operon Biotechnologies).

Proprietary wash buffers from Rheonix with reduced EtOH concentrations were also successfully used to replace the wash buffers provided with the High Pure Viral RNA kit.

A 1 ml mixture composed of 200 µl of crude DNA (20 µg DNA maximum), 450 µl water and 350 µl S3 buffer + RNAse (buffers provided with the kit) was passed through the column (step 3).

The samples were incubated at 70°C for 15 minutes, and then chilled to room temperature, neutralized with 4.5 μl 1 M HCl and purified using the MinElute Reaction Cleanup system (Qiagen), following the manufacturer's recommendations, except that the wash and elution buffers provided with the system were replaced by 80% ethanol and 100 mM NaHCO3 (pH 9.0), respectively.

Amplified libraries were purified using 30 to 50 μl AMPure XP beads (Beckman-Coulter, Brea, CA, USA), and eluted off the beads in 52.5 μl resuspension buffer (provided with Nextera XT).