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The cells were collected, washed with phosphate-buffered saline, and lysed by the specific lysis buffer provided by the kit.
Cells were harvested and homogenized with the lysis buffer provided by the kit at 4°C.
Experiments carried out in serum and in phosphate buffer provided the apparent rate constants for the enzymatic and non-enzymatic oxygen-generation reactions.
ATPS comprising PEG 600 phosphate buffer provided a means for the recovery of proteins from crude extracts of C. brasilienses seeds.
Briefly, samples were fixed and incubated with Sirius Red solutions for 30 min at room temperature, and eluted using extraction buffer provided in the kit.
By destroying soil nutrients through the overuse of chemical fertilizers and pesticides, conventional agriculture jeopardizes food security and nutrition, and reduces ecosystems' resiliency to flood and drought by removing the protective buffer provided by soil's organic carbon.
The supernatant was removed and nuclei were washed and resuspended in storage buffer (provided).
Thereafter, cells were rinsed once in 1× rinsing buffer provided in the kit.
Note: (1) TRIzol® Reagent was substituted for lysis buffer provided in extraction kit.
For the elution, water was used instead of the buffer provided by the kit.
Glass beads were added to the feces with sterile buffer (provided in Qiagen kit) and mixed for 90s.
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