Sentence examples for buffer proteins were subjected from inspiring English sources

After the beads were washed twice with the cell lysis buffer, proteins were subjected to SDS-PAGE.

After washing twice with NET+1% NP-40 and twice with NET buffer, the precipitated proteins were subjected to Western blotting.

After 1 h incubation with glutathione beads (Amersham), complexes were washed 3 times with binding buffer, and the bound proteins were subjected to western analysis.

After 1 h incubation with streptavidin beads (Amersham), complexes were washed 3 times with binding buffer, and the bound proteins were subjected to western analysis.

After being washed three times with the lysis buffer or PBS, the proteins were subjected to immunoblotting analysis.

The beads were washed three times with the lysis buffer, and the bound proteins were subjected to SDS-PAGE (10% gel) and western blotting (Nagata et al, 2009).

The proteins were eluted with elution buffer (Qiagen) and the eluted proteins were subjected to 12% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and immunoblotted with anti-subolesin antibodies [ 8].

After this pull-down reaction, beads were washed with binding buffer and κB probe-bound proteins were subjected to 9% SDS PAGE and immunoblotted with the following antibodies: p65, p50, Bcl-3 (Santa Cruz), p100/52 (Cell Signaling), and Hsc70 for the same blots.

Forty-eight hours after siRNA transfection, FHCC98 and 7721 cells were harvested in a lysis buffer and equal amounts of cellular proteins were subjected to SDS-PAGE (10%).

FHCC98 and 7721 cells were harvested in a lysis buffer, and equal amounts of cellular proteins were subjected to SDS-PAGE (10%).

All reactions were quenched by addition of 2x sample buffer and incubated at 95°C for 5 min. Proteins were subjected to immunoblot analyses.