Sentence examples for buffer proteins were resolved from inspiring English sources

After elution with 20 µl 2× SDS loading buffer, proteins were resolved by SDS-PAGE and analyzed by Western blotting with anti-HA antibody to detect ubiquitination, and anti-WRN antibody to detect WRN protein level.

After the final wash, the pellet was resuspended in sample buffer, and proteins were resolved by SDS-PAGE (4 12% Bis-Tris NuPAGE gel) and transferred to a PDVF membrane for immunoblot analysis using HRP-conjugated V5 or Myc antibodies.

The pellet was resuspended in a 2x Laemmli sample buffer and proteins were resolved by SDS-PAGE and immunoblotted forBcl-2 and Bcl-xL as above.

Beads were mixed with 30 µl of Laemmli blue buffer, and proteins were resolved on a 12 SDS-PAGE and transferred onto PVDF transfer membrane.

Pellets and supernatants were mixed with Laemmli blue buffer and proteins were resolved on a 12% SDS-PAGE and transferred onto PVDF transfer membrane.

Reactions were stopped by adding 5X sample buffer, and proteins were resolved by SDS-PAGE.

Beads were boiled in sample loading buffer and proteins were resolved on a reducing SDS-PAGE gel.

Bound gephyrins were released by boiling in SDS‐loading buffer, and proteins were resolved on a 6% SDS PAGE.

The dried material was dissolved in 10 μl loading buffer, and proteins were resolved by SDS-PAGE on 14% polyacrylamide gel for 250 Vh.

Bacterial pellets recovered after inductions were dissolved in appropriate volume of Laemmli buffer and proteins were resolved on a 12% SDS-PAGE.

Elution was performed using Laemmli buffer, and proteins were resolved on SDS-PAGE, followed by western blotting analysis with appropriate antibodies.