Sentence examples for buffer proteins were removed from inspiring English sources

After washing the beads with cold RIPA buffer, proteins were removed from the beads in 40 μl 2× Laemmlli Sample buffer and analyzed by SDS-PAGE and western blotting.

The resin was washed with KLB buffer and proteins were removed from the resin by the addition of 50 μl undiluted SDS-loading buffer.

The resin was washed with lysis buffer, and proteins were removed from the resin by the addition of 100 μl undiluted SDS-loading buffer.

PCT cells or renal tissues were homogenized in an ice-cold GSH buffer, and proteins were removed by precipitation using perchloric acid and centrifugation.

The beads were washed three times in IPP150 buffer, then bound proteins were removed by cleavage with TEV protease (Invitrogen) for 1.5 h at room temperature in a buffer composed of 10 mM Tris-Cl pH 8.0, 150 mM NaCl, 2% CHAPS, 0.5 mM EDTA and 1 mM DTT.

After loading 25 ml of sample, the column was washed with the same buffer until unbound proteins were removed.

Agarose pellets were washed three times in detergent lysis buffer, and the bound proteins were removed by boiling in 1 × Laemmli buffer.

Washing was performed with the lysis buffer four times after binding, and the proteins were removed from the resin in SDS sample buffer.

The contaminant proteins were removed with wash buffer containing 50 mmol/L imidazole, and subsequently the 6× His-tagged FadR proteins in three versions (FadR_she, FadR_ec and FadR_vc) were eluted in elution buffer containing 100 mmol/L imidazole.

The unbound proteins were removed by washing with buffer (20 mmol/L Tris-HCl pH 8.0 and 200 mmol/L NaCl) for 5 times.

Low-strength, metal-binding proteins were removed by washing with buffer-A containing 50 mM imidazole.