Sentence examples for buffer proteins were heated from inspiring English sources

After suspending 50 µg of cell extract in SDS sample buffer, proteins were heated at 95°C for 5 min and separated by 4 12% SDS-polyacrylamide gel electrophoresis.

The purified proteins were heated to 68°C for 5 minutes, cooled and diluted into reaction buffer containing substrate.

After resuspension in 4× SDS sample buffer, the protein extracts were heated at 100°C for 5 min and subsequently resolved by SDS-PAGE in a 7.5% or 12% acrylamide gel.

Proteins in sample buffer were heated at 37°C, resolved by SDS-polyacrylamide gel electrophoresis in 10% acrylamide gels using tricine buffer, and transferred to nitrocellulose membranes.

Equal amounts of proteins mixed with Laemmli buffer were heated up to 95°C for 5 min and run on sodium dodecyl sulphate 10%-polyacrylamide gels before blotting on polyvinyldifluoride (PVDF) membranes (WESTRAN® clear signal; GE Healthcare Europe GmbH, Freiburg, Germany).

Proteins from the lysates were solubilized in 4× SDS loading buffer and were heated for 5 min at 100°C.

Samples (50 µg protein in 30-µl Laemmli buffer) were heated at 95°C for 5 min, resolved by 10% SDS-PAGE (50 µg/lane), and electro-transferred to PVDF.

Mouse or human protein samples in SDS sample buffer were heated to 95 °C for 10 min and separated on SDS-polyacrylamide gels.

Then, 50 µg of protein were heat denatured in SDS-PAGE sample buffer and resolved on denaturing (SDS) 10% polyacrylamide gels (SDS-PAGE).

Protein samples were heated at 95°C with SDS-PAGE sample buffer and analyzed on a SDS-PAGE gel.

Protein lysates were heated to 95 °C for 5 min in Laemmli buffer.