Sentence examples for buffer proteins were fractionated from inspiring English sources

The NaOH was neutralized by the addition of 5 µL of 4 M acetic acid and after the addition of Laemmli buffer proteins were fractionated by SDS polyacrylamide gel electrophoresis and analyzed by immunoblotting using anti-HSF1 (Neef et al. 2010) and anti-PGK1 (22C5D8; Abcam) antibodies.

Cells were harvested at indicated time points, lysed in reducing Laemmli buffer and proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) according to standard procedures [33].

Western blots were carried out by lysing transfected cells (30 μl) in 1× Laemmli SDS-PAGE loading buffer [ 68] and proteins were fractionated on a 10% SDS-PAGE gel with a constant current of 35 mA.

Protein solutions were buffer exchanged and per sample 50 μg of proteins were fractionated on a 4-12% gradient gel and lanes fractionated into 20 pieces.

Beads were washed four times and bound proteins were fractionated by SDS-polyacrylamide gelectrophoresissis.

Proteins were fractionated in 10% SDS/polyacrylamide gel electrophoresis and then transferred to a nitrocellulose membrane.

In-gel trypsin digested proteins were fractionated on microcapillary RP-C18 (Ciborowski et al., 2004).

First, purified His6-tagged proteins were fractionated by HPLC as described [17].

Proteins were fractionated in SDS polyacrylamide gels.

The proteins were fractionated on 4 12% NuPAGE pre-cast SDS-PAGE gels at 175 V for 45 min using MES-SDS running buffer.

Nuclear proteins were fractionated by 10% SDS-PAGE.