Sentence examples for buffer proteins were eluted from inspiring English sources

After washing with lysis buffer, proteins were eluted from beads by boiling in Laemmli buffer for 5 min at 95°C.

After 3 washes with ice-cold lysis buffer, proteins were eluted in reducing Laemmli's buffer, resolved by SDS-PAGE, transferred to nitrocellulose and immunoblotted with the indicated antibodies.

Subsequent to washing in low salt buffer, proteins were eluted with a linear gradient developed over 40 min at 1 ml/min into high salt buffer (20 mM Tris-HCl, 0.5 M NaCl, pH 8.5).

After 3 washes in lysis buffer, proteins were eluted from the beads in reducing Laemmli's buffer, resolved by SDS-PAGE, transferred to nitrocellulose and analysed by western blot with the indicated antibodies.

Each column was rinsed four times with 500 µl IP buffer; proteins were eluted with 30 µl 'sample buffer' (Cytosignal) and incubated at room temperature for 15 minutes before centrifugation at 20,000×g for 2 minutes.

After five washes with RIPA buffer, proteins were eluted and analyzed by SDS PAGE.

After wash with lysis buffer, protein was eluted with lysis buffer containing 200 mM imidazole.

After washing with 10 vol of IE buffer, protein was eluted with IE buffer + 400 mM NaCl and was further purified on a Superdex 200 size-exclusion column (SEC) in 100 mM NaCl, 10 mM NaF, 10 mM (4- 2-hydroxyethyl -1-piperazineethanesulfonic acid [HEPES]), pH 7, 5 mM DM.

Beads were washed twice with wash buffer and proteins were eluted in elution buffer (same as lysis buffer but with 250 mM imidazole).

The supernatant was purified by Ni Sepharose (GE) affinity chromatography equilibrated with buffer A. Proteins were eluted using buffer A supplemented with 250 mmol/L imidazole.

After washing the beads with the corresponding buffer, bound proteins were eluted with buffer lacking Ca2+ ions.