Sentence examples for buffer prior to staining from inspiring English sources

RBCs were lysed with ammonium chloride buffer prior to staining.

For intracellular staining cells were fixed with 1% formaldehyde for 10 min and subsequently permeabilized with 0.5% saponin in 1% BSA FACS buffer, prior to staining with anti-TLR 9, TLR 3 or IL-12 antibody.

For peripheral blood analysis, tailvein blood was collected and RBCs were lysed with ammonium chloride buffer prior to staining for flow cytometry.

Colon tissue was removed, dissected longitudinally, flushed with phosphate-buffered saline (PBS), fixed in 10% (v/v) neutral buffered formalin prior to staining with hematoxylin and eosin (H&E).

For antigen retrieval, sections were heated in a pressure cooker in 10-mM citrate buffer (pH 6.0) prior to staining.

Horizontal sections (40 µm) through the hippocampus were cut from a dry ice-cooled block on a sliding microtome (Leica, Deerfield, IL) and stored in 0.1 M phosphate buffer (pH 7.4) prior to staining.

Cells were then either taken for staining or permeabilized using a Cytofix/Cytoperm buffer solution (BD Biosciences, San Jose, CA, USA) prior to staining.

Antibodies were diluted 1 20 with Flow Cytometry Staining Buffer (BD Biosciences, Cat. No. 00-4222-26) prior to staining.

For immunohistochemistry, all sections were, fixed in acetone and methanol (1 1) or 4% paraformaldehyde (for MyoD), and subsequently blocked in buffer (3% fetal calf serum in PBS) prior to staining.

Briefly, tissues were washed in phosphate buffered saline and incubated in blocking buffer (1% BSA, 0.3% Triton X-100, 1 mM CaCl2) prior to staining with antibodies to BrdU[ 23] (a gift from Dr. Jeffrey Gordon, Washington University School of Medicine, St . Louis MO; 1 1000) and detected with fluorescent secondary antibodies.

Prior to staining, 220 μl 1 × MES buffer containing 2 mg/ml acetylated BSA and 2 μg/ml biotinylated anti-streptavidin antibody (Vector) was applied onto the array for 30 min at 40°C.