Sentence examples for buffer prior to separation from inspiring English sources

The input bacterial extract sample and 1M NaCl eluates were precipitated by methanol-chloroform and re-solubilized in SDS-sample buffer prior to separation by SDS-PAGE.

The powder was resuspended in 2× Novex Native TrisGly sample buffer (Invitrogen) prior to separation in 4% Novex Tris-Glycine gel.

The magnetic beads with immobilized RNA and its bound proteins were washed three times with the binding buffer and bound proteins were eluted by boiling 3 min the in sample buffer prior to 4 12 % SDS-PAGE (NuPAGE 4 12 % Bis Tris Gel, Invitrogen) separation and silver staining (SilverQuest, Invitrogen).

Beads were washed three times with lysis buffer prior to use.

Positive ccs were buffer-exchanged into carbonate buffer prior to purification using a stirred ultrafiltration cell (Amicon) or by dialysis.

After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.

Beads were washed twice with HEPES buffer and once with lyses buffer prior to incubation with cell extracts.

Beads were washed 3 X in PB buffer and resuspended in 2X Laemelli sample buffer prior to western blotting.

RBCs were lysed with ammonium chloride buffer prior to staining.

Protease inhibitors were added to all buffers excluding the urea buffer prior to use.

Deglycosylated ovalbumin was buffer exchanged into aqueous AmAc buffer prior to CIP dephosphorylation.