Sentence examples for buffer prior to purification from inspiring English sources

For KatA1-49 the pellet containing the insoluble fraction was dissolved in binding buffer with 8 M urea and proteins were then refolded by dialysis against binding buffer prior to purification.

Positive ccs were buffer-exchanged into carbonate buffer prior to purification using a stirred ultrafiltration cell (Amicon) or by dialysis.

The reaction mixture was neutralised by the addition of sodium phosphate buffer (pH 7.2) prior to purification by high-performance liquid chromatography (HPLC), which was performed over a semipreparative Alltima C18 reverse-phase column (5  μm, 10 × 250 mm. Alltech, Deerfield, IL, USA) using 3% EtOH, 10 m M NaH2PO4 in saline as the eluent (flow: 5 ml min−1, R t : 16 min).

Eluted protein was precipitated by TCA precipitation and dissolved into SDS sample buffer prior to resolution in a polyacrylamide gel.

Precipitated protein was dissolved in sodium dodecyl sulfate (SDS) sample buffer prior to resolution in a polyacrylamide gel.

RBCs were lysed with ammonium chloride buffer prior to staining.

Protease inhibitors were added to all buffers excluding the urea buffer prior to use.

Deglycosylated ovalbumin was buffer exchanged into aqueous AmAc buffer prior to CIP dephosphorylation.

Cells were washed 3-4 tines in phosphate buffered saline (PBS) at room temperature prior to purification of CD4+ T cells by positive selection using anti-human CD4 microbeads (Miltenyi Biotec).

This whey was filtered to 0.45 µm prior to purification using Protein-G Sepharose chromatography, using PBS as running buffer and 50 mM citrate pH 2.6 as elution buffer.

Monomeric protein was produced by adding n-dodecyl-β D-maltopyranoside (β-DDM) to the supernatant (and all buffers) to a final concentration of 0.05% and gently incubating for 1 min in a sonicating water bath prior to purification.