Sentence examples for buffer prior to protein from inspiring English sources

The sample buffer prior to protein concentration contained 10 mM Tris, pH = 7.5, 100 mM NaCl, and 10 mM dithiothreitol.

Cells contained in 1 mL of culture broth were harvested by centrifugation (4000×g at 4 °C for 20 min) and resuspended in Laemmli sample buffer, prior to being analyzed for total protein extract by 10%% SDS PAGE following the Laemmli method (Laemmli 1970).

Insoluble material was pelleted at 10 000 g for 10 min. Supernatants were stored in Laemmli protein sample buffer prior to electrophoresis in gradient gels (Bio-Rad, Invitrogen).

The beads were heated at 100°C for 5 min with 80 μl of protein sample buffer prior to SDS-PAGE and immunoblotting.

Such limited colocalisation with Dp persisted in cells treated with CSK buffer prior to fixation, a procedure that removes proteins not associated with the cytoskeleton (Data not shown).

The supernatants were solubilized 1 3 in 4X concentrated Laemmli buffer prior to loading and analysis for nuclear proteins by WB.

For the quantification, total protein concentrations in expectorations were measured using Pierce BCA protein assay and then solubilized with Laemmli buffer prior to electrophoresis.

Fractions containing protein were mixed with SDS loading buffer prior to SDS-PAGE analysis.

Precipitated protein was dissolved in sodium dodecyl sulfate (SDS) sample buffer prior to resolution in a polyacrylamide gel.

For KatA1-49 the pellet containing the insoluble fraction was dissolved in binding buffer with 8 M urea and proteins were then refolded by dialysis against binding buffer prior to purification.

Following incubation at 50°C and washing, beads were recovered and bound proteins eluted by boiling in SDS-PAGE loading buffer prior to western blotting.