Sentence examples for buffer prior to loading from inspiring English sources

Beads were washed 2× with kinase buffer, and then boiled in 2× SDS-PAGE loading buffer, prior to loading on the gel and subsequent Western blotting.

All strips were washed with 2×electrophoresis buffer prior to loading on the second dimension gel and sealed with 2×electrophoresis buffer containing 0.5% low melting agarose.

Sequencing mixes were diluted to the required concentration with the manufacturer provided dilution buffer prior to loading onto 96-well plates.

The supernatants were solubilized 1 3 in 4X concentrated Laemmli buffer prior to loading and analysis for nuclear proteins by WB.

Reactions were incubated for 30 min at RT and quenched with 10 μL 2× SDS-PAGE sample loading buffer prior to loading onto 10% stacking gels.

Supernatant fractions were collected, and pellets were dissolved in electrophoresis sample buffer prior to gel loading.

A Ni2+-Sepharose 6 Fast Flow resin (GE Healthcare) resin was equilibrated with 5 CVs (column volumes) of binding buffer prior to sample loading.

Samples were automatically loaded across a Paradigm Platinum Peptide Nanotrap (Michrom) pre-column (0.15 x 50 mm, 400 µl volume) for sample concentrating and desalting, at a flow-rate of 50 µl/min in HPLC buffer A prior to loading into an inline analytical capillary column (75 µm x 12 cm) with C18 resin (5 µm, 200A° Magic C18AG, Michrom) and Picofrit capillary tubing (New Objective, Cambridge, MA).

Where necessary, sample dilutions were carried out in Laemmli sample buffer (BioRad Laboratories) prior to loading on the gel.

The samples were boiled at 100°C for 5 min in a reducing buffer (125 mM Tris-HCl; 140 mM SDS; 20% (v/v) glycerol; 200 mM dithiothreitol (DTT) and 30 mM bromophenol blue) or left at room temperature for 5 min in a non-reducing buffer (as for reducing buffer minus DTT) prior to loading.

The cells were then lysed in 1 ml SDS-PAGE loading buffer prior to PAGE and western blotting.