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Agrobacterium cultures were resuspended in infiltration buffer prior to infiltration [ 39].
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Eluted protein was precipitated by TCA precipitation and dissolved into SDS sample buffer prior to resolution in a polyacrylamide gel.
Precipitated protein was dissolved in sodium dodecyl sulfate (SDS) sample buffer prior to resolution in a polyacrylamide gel.
For KatA1-49 the pellet containing the insoluble fraction was dissolved in binding buffer with 8 M urea and proteins were then refolded by dialysis against binding buffer prior to purification.
Beads were washed three times with lysis buffer prior to use.
Positive ccs were buffer-exchanged into carbonate buffer prior to purification using a stirred ultrafiltration cell (Amicon) or by dialysis.
After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.
Beads were washed 3 X in PB buffer and resuspended in 2X Laemelli sample buffer prior to western blotting.
Beads were washed twice with HEPES buffer and once with lyses buffer prior to incubation with cell extracts.
BCCP and BPL were dialyzed against the standard buffer prior to use.
RBCs were lysed with ammonium chloride buffer prior to staining.
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