Sentence examples for buffer prior to fixation from inspiring English sources

Such limited colocalisation with Dp persisted in cells treated with CSK buffer prior to fixation, a procedure that removes proteins not associated with the cytoskeleton (Data not shown).

The samples were rinsed 2×10 minutes in 0.1 M sodium cacodylate buffer prior to post-fixation (2% OsO4 in 0.1 M sodium cacodylate buffer) for 1 hour, and rinsed 5×10 minutes in distilled water before bulk staining with 1.5% uranyl acetate [(CH3COO 2UO2·2H2O] in distilled water for 30 minutes in the dark.

Cells were cultured on coverslips and washed with phosphate buffered saline (PBS) prior to fixation.

RBCs were lysed with ammonium chloride buffer prior to staining.

Protease inhibitors were added to all buffers excluding the urea buffer prior to use.

Deglycosylated ovalbumin was buffer exchanged into aqueous AmAc buffer prior to CIP dephosphorylation.

The same procedure was employed to stain MKs, except that cell suspensions of 5×10 cells mL−1 were employed for incubation and spreading was allowed in medium (and not buffer) for 3 h prior to fixation and mounting.

Cells were then transfected as described and then stimulated with the indicated treatments prior to fixation with fixing buffer (3% (w/v) paraformaldehyde, 1% (w/v) sucrose, 1 mM CaCl2, 1 mM MgCl2 in PBS (37 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 1.46 mM KH2PO4, pH 7.4)).

The prostate was weighed, measured, and inked prior to fixation in 10%% buffered formaldehyde.

For the uH2A immunostaining cells were permeabilized prior to fixation by in cytoskeletal buffer (100 mM NaCl, 300 mM sucrose, 3 mM MgCl2, 10 mM PIPES pH6.8) for 30 sec, followed by 30 sec in cytoskeletal buffer containing 0.5% Triton-X100, and 30 sec in cytoskeletal buffer without Triton-X100, all on ice.

For endogenous SLX4 immunofluorescence, cells grown on poly-L-lysine-coated coverslips were preextracted in CSK buffer or PBS containing 0.5% Triton X-100 prior to fixation in 3% PFA in CSK (pH 7.0).