Sentence examples for buffer prior to dehydration from inspiring English sources

The fixed MPT were treated with 1% osmium tetroxide (Sigma) in 0.1 M cacodylate buffer prior to dehydration in increasing concentrations of ethanol (30 to 100%).

Samples were washed thoroughly with fixative buffer prior to dehydration with a graded ethanol series (50, 70, 80, 90, 95, and 2 × 100% (v/v) ethanol for 10 min each).

This "tuning up" of an enzyme's pH is a particularly important step in non aqueous enzymology, and it is often accomplished while the enzyme is dissolved in a buffer prior to dehydration (usually by lyophilization) before suspension in the organic solvent of choice [ 9].

Beads were washed three times with lysis buffer prior to use.

Positive ccs were buffer-exchanged into carbonate buffer prior to purification using a stirred ultrafiltration cell (Amicon) or by dialysis.

After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.

Beads were washed twice with HEPES buffer and once with lyses buffer prior to incubation with cell extracts.

Beads were washed 3 X in PB buffer and resuspended in 2X Laemelli sample buffer prior to western blotting.

BCCP and BPL were dialyzed against the standard buffer prior to use.

RBCs were lysed with ammonium chloride buffer prior to staining.

Protease inhibitors were added to all buffers excluding the urea buffer prior to use.