Sentence examples similar to buffer prior to assays from inspiring English sources

Cell number was counted using a Neubauer chamber and adjusted to 5×1010 cells/ml in 1X Apoptosis Wash Buffer, prior to assays using the MultiCaspase Detection Kit (Guava Technologies).

The number of adherent cells was determined prior to assays.

Two types of purified ribosome preparations were used: we either used an aliquot of the ribosome containing fraction eluted from the QA column, or we concentrated the eluted ribosomes by centrifugation - 2 h at 31,000 rpm at 4°C, in a Beckman MLS-50 rotor (Beckman-Coulter) using an Optima Max tabletop ultracentrifuge (Beckman-Coulter) and resuspended them in ribosomal buffer prior to the assay.

Prior to assay, cells were washed twice with the XF assay buffer (unbuffered DMEM supplemented with 25 mM glucose and 10 mM sodium pyruvate, pH 7.4).

The thermal stability of SOD was determined by incubating the purified SOD in 50 mM phosphate buffer (pH 7.8) at a series of elevated temperatures (25 60°C) for 60 min prior to assay for SOD activity.

Prior to assay, adrenal fragments were pre-incubated in 0.5 ml of the above buffer (50 100 mg of adrenal tissue per ml) in a water bath for 5 min at 37°C with continuous gentle shaking.

A total of 100 μl of buffer was placed in to each well and the plates were put in a 37°C incubator for an additional 30 minutes prior to assay.

ABTS solution was prepared freshly prior to assay.

Cells were incubated for the indicated times prior to assay.

Frozen AFs were centrifuged and aliquoted prior to assay.

The sample supernatants were reserved at −80°C prior to assay.