Sentence examples for buffer prior to analysis from inspiring English sources

An aliquot of 70 μL was diluted to 280 μL (1 4) with flow cytometry buffer prior to analysis if running immediately or 2% formaldehyde if stored overnight at 4°C. Cell suspensions were analyzed by flow cytometry on a FACS Calibur flow cytometer.

The rhLF solution (1 mg/ml) was treated with PNGase F (final concentration of 5 U/µl) and Endo H (final concentration of 5 U/µl) respectively, and successively boiled for 5 minutes in non-reducing SDS-PAGE sample buffer prior to analysis by 7.5% SDS-PAGE.

Specifically, all serum samples from all calves were diluted 1 2,000 in sample buffer prior to analysis.

Serum samples were diluted 1 50 (Chlorocebus aethiops only) or 1 100 (all others) with wash buffer prior to analysis.

Cells were incubated with fluorophore conjugated antibody for 30 min at 4 °C and washed once with PBS buffer prior to analysis.

Cells were rapidly washed in ice-cold PBS and directly lysed in preheated LDS sample buffer, prior to analysis by western blot.

Reaction was performed at 30°C for 30 minutes and quenched with Laemmli sample buffer prior to Western analysis.

Beads were collected by centrifugation and washed four times with lysis buffer prior to gel analysis.

After centrifugation the supernatant was dissolved in SDS loading buffer prior to immunoblot analysis.

Cells were washed twice in PBS and incubated for 1 h (4 °C) with fluorescent labelled secondary antibodies diluted in PBS/0,5 % BSA (FACS buffer), washed twice and resuspended in FACS buffer prior to FACS analysis.

The immunoprecipitated proteins were washed at 4°C in the lysis buffer prior to direct analysis by SDS-PAGE (8% acrylamide gel) and blotted onto a nitrocellulose membrane (Bio-Rad).