Sentence examples for buffer prior to analyses from inspiring English sources

The immune complexes were released by boiling 5 min at 95°C in Laemmli sampling buffer prior to analyses by Western.

To quantify bacteria in hemolymph samples, 0.5 μl of hemolymph (collected as described for metabolite analyses) was diluted in 300 μl cell lysis buffer prior to DNA extraction and qPCRs as previously described (Herren and Lemaitre, 2011).

Beads were washed three times with lysis buffer prior to use.

Positive ccs were buffer-exchanged into carbonate buffer prior to purification using a stirred ultrafiltration cell (Amicon) or by dialysis.

After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.

Beads were washed twice with HEPES buffer and once with lyses buffer prior to incubation with cell extracts.

Beads were washed 3 X in PB buffer and resuspended in 2X Laemelli sample buffer prior to western blotting.

BCCP and BPL were dialyzed against the standard buffer prior to use.

RBCs were lysed with ammonium chloride buffer prior to staining.

Protease inhibitors were added to all buffers excluding the urea buffer prior to use.

Deglycosylated ovalbumin was buffer exchanged into aqueous AmAc buffer prior to CIP dephosphorylation.