Sentence examples for buffer prior to addition from inspiring English sources

After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.

After washing, the cells were blocked with various concentrations of AAL-biotin B-13955, Vector Labs) or kept in buffer prior to addition of antibodies.

Ni2+-NTA spin columns (Qiagen, ON, Canada) were equilibrated in dialysis buffer prior to addition of the S100B/IC3 mixture (600 μL).

After centrifugation, the cell lysate was collected and diluted 1 1 with KPE buffer prior to addition of freshly prepared DTNB and GSH reductase solutions, prepared according to [ 17].

Cells were incubated for 1 hour with FLIPR loading buffer prior to addition of ligand and fluorescent intensity was measured at 37°C using a Flexstation 3 (Molecular Devices).

Arrays were then washed three times in ice cold probing buffer prior to the addition of streptavidin-alkaline phosphatase (Invitrogen) diluted in probing buffer to a final concentration of 0.25 µg/ml.

ELISA plates were rinsed seven times with an excess of PBS-T buffer prior to the addition of 100 µl secondary antibody diluted 1∶2500 in PBS-T buffer; the secondary antibody was alkaline phosphatase-conjugated rabbit anti-mouse γ chain (Rockland Immunochemicals, Gilbertsville, PA).

Beads were washed in IPP buffer prior to the addition of 120 μl (S methionine-labelled K562 cell extract in IPP buffer.

Per reaction, 1 μg substrate was added in 20 μl kinase buffer prior to the addition of 10 μl ATP mix (40 mM MnCl2, 80 μM ATP, 1 μCi P γ-ATP in kinase buffer).

Cells were washed once with fresh FACscan buffer prior to the addition of primary antibodies or isotype controls and stained overnight at 4°C in accordance to the manufacture's guidelines.

Although previous work had shown that the antigen-antibody interactions are typically optimum at pH > 7 [ 24], fungal derived enzymes are typically buffered and used at around pH < 5. We therefore brought the enzyme samples up to pH 7.5 using PBS buffer prior to their addition to the wells.