Sentence examples for buffer prior to acidification from inspiring English sources

The trypsin homogenate was centrifuged and washed with ABC buffer prior to acidification with 10% formic acid.

Beads were washed three times with lysis buffer prior to use.

Beads were washed 3 X in PB buffer and resuspended in 2X Laemelli sample buffer prior to western blotting.

Positive ccs were buffer-exchanged into carbonate buffer prior to purification using a stirred ultrafiltration cell (Amicon) or by dialysis.

After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.

Beads were washed twice with HEPES buffer and once with lyses buffer prior to incubation with cell extracts.

BCCP and BPL were dialyzed against the standard buffer prior to use.

RBCs were lysed with ammonium chloride buffer prior to staining.

Protease inhibitors were added to all buffers excluding the urea buffer prior to use.

Deglycosylated ovalbumin was buffer exchanged into aqueous AmAc buffer prior to CIP dephosphorylation.

The supernatant was filtered (0.2 μm) prior to acidification (nitric acid) and analysis of elemental composition.