Sentence examples for buffer placed on a from inspiring English sources

After 30 min, the samples were washed with wash buffer, placed on a rocker for 10 min, and washed again.

The cells were passed through a MACS-LS column equilibrated with PBE buffer placed on a Midi-Macs Magnet (Myltenyi Biotech Inc., Auburn, CA, USA), and the CD133 negative cells in the flow-through were collected.

The pellets were resuspended in cytoplasmic lysis buffer, placed on ice for 10 minutes, vortexed for 30 seconds, and pelleted.

Cells were then washed, resuspended (20x10 cells/ml) in FACS buffer, placed on ice, and labeled for 20 minutes with PE-conjugated anti-HSA (clone M1/69; 1 750 dilution), biotinylated anti-CD23 (clone B3B4; 1 200 dilution) which was visualized by PerCP-Cy5.5-conjugated streptavidin (1 200 dilution), and APC-conjugated anti-C1qRp (clone AA4.1; 1 100 dilution) for B cell subset identification.

The cell suspension in PBS buffer was placed on a glass slide to obtain optic (left) and fluorescent (right) images.

A drop of M9 buffer was placed on a clean glass slide.

48 hours after electroporation, axolotl cells were rinsed twice with 0.7× PBS, lysed with 250 μl of fresh 1× passive lysis buffer and placed on a shaker with gentle agitation for 20 minutes.

Plugs were rinsed three times with 1 × TE buffer and placed on a shaker for 5 min. The last step was repeated once and three more washes were performed with intervals of 10, 15 and 20 min. The solution was replaced by 150 μL of buffer A (New England Biolabs, ON, Canada) and after 10 min replaced by buffer A plus 60 U of restriction enzyme SmaI and incubated at 25°C overnight.

After incubation, test animals were washed again in PBS buffer and then placed on a glass slide.

After sonication, 20 µL of SWCNT in 25 mM HEPES buffer (pH 7.4) was placed on a freshly cleaved mica which was subsequently rinsed with DI water while being spun at 950 rpm to remove the excess of the buffer solution.

All slides were rinsed in Tris buffered saline (TBS), placed on an autostainer and washed in TBS followed by 3%H2O22 in TBS for 5 minutes.