Sentence examples for buffer placed on from inspiring English sources

After 30 min, the samples were washed with wash buffer, placed on a rocker for 10 min, and washed again.

The pellets were resuspended in cytoplasmic lysis buffer, placed on ice for 10 minutes, vortexed for 30 seconds, and pelleted.

The cells were passed through a MACS-LS column equilibrated with PBE buffer placed on a Midi-Macs Magnet (Myltenyi Biotech Inc., Auburn, CA, USA), and the CD133 negative cells in the flow-through were collected.

Cells were then washed, resuspended (20x10 cells/ml) in FACS buffer, placed on ice, and labeled for 20 minutes with PE-conjugated anti-HSA (clone M1/69; 1 750 dilution), biotinylated anti-CD23 (clone B3B4; 1 200 dilution) which was visualized by PerCP-Cy5.5-conjugated streptavidin (1 200 dilution), and APC-conjugated anti-C1qRp (clone AA4.1; 1 100 dilution) for B cell subset identification.

The system consists of a metallic shell which is inserted directly on the acetabulum and a PCU buffer insert placed on the inside of the shell (buffer-with-shell configuration).

After the incubation, yeast were washed three times with 100 µL of FACS buffer, then resuspended in 200 µL of FACS buffer and placed on ice.

The cell suspension in PBS buffer was placed on a glass slide to obtain optic (left) and fluorescent (right) images.

The cells in the 1× RIPA buffer were placed on ice for 30 min with occasional vortexing.

Harvested cells were immediately seeded into PCR tubes containing reverse transcription buffer, and placed on ice.

A drop of M9 buffer was placed on a clean glass slide.

In brief, worms grown on NGM plates were washed with M9 buffer and placed on 48 h-old C. albicans lawns (on BHI agar plates) for 2 h.