Sentence examples for buffer placed from inspiring English sources

After 30 min, the samples were washed with wash buffer, placed on a rocker for 10 min, and washed again.

The pellets were resuspended in cytoplasmic lysis buffer, placed on ice for 10 minutes, vortexed for 30 seconds, and pelleted.

Worms were washed three times in M9 buffer, placed in 1% SDS for 15 min [19], and then checked for survival.

In order to reduce LF82 carryover, nematodes were washed twice in 500 µl of M9 buffer placed in glass depression slides before placing on OP50/pQW58 lawns.

After incubating with gentle agitation for 16 20 hours, specimens were removed from the buffer, placed in 100%EtOH for 2 4 hours to stop further digestion, air-dried, and replaced in their collections.

After washing with a solution of 10 mM Tris HCl, 133 mM NaCl, and 0.2% Tween 20 (TBST), the secondary Ab diluted 1∶5000 was added to the reaction, with membranes being subsequently washed 4 times for 5 min, equilibrated in luminescence buffer, placed in chemiluminescent substrate (Roche, Switzerland), and finally exposed to an X-Ray film.

3.75ml/200= 18.75 μl in 3.731ml mix buffer Place the solution in a small falcon tube that should be kept cold.

In contrast, previous distance-threshold investigations have used arbitrarily sized buffers placed upon predetermined locations in response to environmental attributes, without considering the combined influence of additional social burdens.

This device includes a data logger, impedance pH amplifiers, and a catheter consisting of two antimony pH sensors (calibrated before and after recordings were taken using standard pH 7 and pH 1 buffers) placed 5 cm above and 10 cm below the lower esophageal sphincter (LES) and eight impedance electrodes placed 2, 4, 6, 8, 10, 14, 16 and 18 cm above the LES.

In the process, two problems arise: (1) disconnected subsystems can exhibit different behaviors (firing sequences and consequently, the reachable markings) from the original ones, and (2) since the buffer places are essentially shared by more than one subsystem, there must be an agreement among the neighboring local controllers.

The system consists of a metallic shell which is inserted directly on the acetabulum and a PCU buffer insert placed on the inside of the shell (buffer-with-shell configuration).