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To prepare the salivary gland lysate (SGL), the glands were resuspended in PBS containing a 1∶50 dilution of protease inhibitor cocktail set III, EDTA-free (EMD Chemicals, Gibbstown, NJ) at a ratio of 1 µl of buffer per pair of salivary glands.
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The Kids Connect Free and Eat Free program is $425 to $525 a night per pair of rooms, plus tax.
Cambridgeshire spent £262 per pair of firefighting trousers, while Wiltshire paid £147.50.
A ratio of 3 ml of buffer per 1 g of roots was used.
£55 per pair; Liberty of London, Regent Street.
The protein pellet was then dissolved in HENS buffer (1% SDS in HEN buffer; 0.1 mL of HENS buffer per mg of protein in the initial protein sample).
IARC/IACR only allow one tumour (depending on histologic group) per organ or pair of organs per person per lifetime.
A buffer zone was traced around each pair of individuals, within each species.
Our search generated 2,590 pathway pairs (7.7±3.1 genes per pair), which cover 5,284 (56% of 9,376) synthetic lethal interaction pairs involving 689 yeast genes.
This involves four pairs of gears, the ratio per pair commonly being between 6 to 1 and 10 to 1.
The 16 µl reaction mix contained 1 µl DNA template, 1X Taq Buffer, 2 mM MgCl2, 0.2 mM of each dNTP, varying concentrations of primers (see below; one primer per pair was fluorescently labelled), and 0.4 U Taq polymerase per primer pair included in the reaction.
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