Sentence examples for buffer per gram of from inspiring English sources

Cells were pelleted, resuspended in 1 mL of S30 buffer per gram of wet cell mass, and lysed using a high-pressure EmulsiFlex-C50 homogenizer (Avestin) operated at 15,000 20,000 psi.

Insect bodies were crushed in liquid nitrogen using a chilled mortar and pestle and an extraction buffer (0.2 M phosphate buffer pH 7.6 containing 2 mM phenylmethanesulfonylfluoride) was added at a ratio of 3 mL buffer per gram of insect powder.

To measure the levels of TXNIP and Sirt1, tissues were homogenized on ice in 300 μl of lysis buffer per gram of tissue.

The tissues were then homogenized at 4°C in five volumes of homogenizing buffer per gram of tissue using a motor driven glass-Teflon Potter-Elvejhem homogenizer.

The sections were minced and homogenized with 2 ml of homogenization buffer per gram of tissue, using a Teflon-glass homogenizer.

The cell pellet from each of the thiol-containing XTEN precursors (XTEN-1, 2, 3, 4) was resuspended in lysis buffer (20 mM Na-phosphate, pH 8.0, 5 mM imidazole, 500 mM NaCl) at a ratio of 3 mL buffer per gram of pellet and lysed using a dynamic high pressure homogenizer.

Trichoplusia ni (T.ni) cells expressing P-gp were harvested by centrifugation and then resuspended in ice-cold hypotonic homogenization buffer (5 mM Tris, 5 mM TCEP, 40 μM leupeptin, 2 μM pepstatin A, 4 mM benzamidine, 200 μM PMSF, pH 7.4) at a ratio of 5 mL of buffer per gram wet-weight of cells and incubated for 45 min on ice.

Five milliliters of homogenization buffer was used per gram of brain tissue and homogenized using six passes of a motorized Wheaton Teflon pestle tissue grinder.

The tissue was digested with papain (10 mg/g dry weight of tissue) in 0.5 M acetate buffer (20 mL per gram of tissue) pH 5.5, containing 5 μM cysteine and 5 μM di-sodium salt of EDTA at 65 °C for 24 h.

For the isolation of organelles, leaves were cut into small slices (approximately 1 mm× 10 mm) with a razor blade and homogenized in a Polytron for 2 sec five to seven times with 5 ml of a homogenizing buffer per gram fresh weight of leaves.

Lung tissues were homogenised with 10 mL of ice-cold lysis buffer (50 mM phosphate buffer containing 1 mM EDTA) per gram of tissue.