Sentence examples for buffer per g of from inspiring English sources

Use about 10 ml of GST binding buffer per g of wet cells.

The plant material was ground in liquid nitrogen and the tissue was extracted in 1 ml of extraction buffer per g of plant material.

Each specimen was finely diced and the macromolecules contained were sequentially extracted for 3 × 24 h periods at 4°C in the dark with PBS (10 mM disodium phosphate, 0.14 M NaCl, pH 7.4), 4 M GdnHCl - 0.05 M sodium acetate and 4 M GdnHCl - 0.05 M sodium acetate - 1% Triton X-100, using 10 vols of extraction buffer per g of tissue [ 21].

Freshly isolated chondrocytes (50 million cells per mL) were combined with the sterile monomer/peptide/photoinitiator solution described above in phosphate buffered saline (PBS) with 1 mg/g of HA (37 kDa, Lifecore Biomedical).

A ratio of 3 ml of buffer per 1 g of roots was used.

For the purification, frozen cell pellets were thawed on ice and suspended in 50 mM Tris (pH 8.0) buffer containing 500 mM NaCl, 10 mM β-mercaptoethanol (β-ME), 20% (v/v) glycerol, 1.4 μg/mL pepstatin A, and 1 μg/mL leupeptin (Buffer A) at a concentration of 5 mL of lysis buffer per 1 g of cell pellet.

Use 2.5 3 ml of buffer per 1 g of N. benthamiana leaf tissue.

Cell disruption and purification: The cell pellet was thawed in lysis buffer [phosphate buffer (50 m m) containing NaCl (300 m m) and imidazole (10 m m), pH 8.0], with use of 10 mL buffer per 5 g of wet cells.

After being minced and homogenized using 5 ml isolation buffer per 1 g of tissue, the homogenate was centrifuged at 1000  g for 10 min, and then the supernatant was collected and again centrifuged at 10 000  g for 10 min to form a crude mitochondrial pellet.

The thawed cell-paste was suspended in 2 mL of S30 buffer per g CWW, cells were lysed in a homogenizer (EmulsiFlex-C55A; Avestin, Manheim, DE) at 20,000 psi and chilled to 0 4°C using a heat exchanger on the homogenizer outlet.

Cell pellet was resuspended in spheroplast lysis buffer (1 mL buffer per 0.5 g of cell pellet; 0.6 M Sorbitol, 10 mM Tris-HCl pH 7.4, 1 mM PMSF).