Sentence examples for buffer pH6 was performed from inspiring English sources

Antigen retrieval, by heating to 80°C for 20 min in citrate buffer, pH6, was performed for vWF and mac-387 staining.

Equilibration of the strips to the SDS buffer system (6 M urea, 2% wt/vol SDS, 30% vol/vol glycerol, and 0.002% wt/vol bromophenol blue in 50 mM Tris-HCl buffer; pH 8.8) was performed in 2 15-minute incubations: the first in SDS buffer containing 1% wt/vol DTT, the second in SDS buffer containing 2.5% wt/vol iodoacetamide.

After washing in PBS, heat-induced antigen retrieval using citrate buffer (pH 6) was performed.

Upon activation of a CM5 sensor chip with a 1 1 mixture of N-hydroxysuccinimide/N-ethyl-N'- 3-dimethylaminopropyl -carbodiimide hydrochloride, amiN-hydroxysuccinimide/N-ethyl-N'- 3-dimethylaminopropyl -carbodiimideiN-hydroxysuccinimide/N-ethyl-N'- 3-dimethylaminopropyl -carbodiimide mM sodium acetate buffer pH 5.0 was performed to couple approximately 1,200 relative uN-hydroxysuccinimide/N-ethyl-N'- 3-dimethylaminopropyl -carbodiimide

A 50 µL injection of CS-A (100 µg/mL) dissolved in 20 mM phosphate buffer pH 7.0 was performed in one flow cell followed by quenching with 1 M ethanolamine hydrochloride pH 8.5, whereas the other flow cell served as control and was quenched by ethanolamine after activation.

The microwave antigen retrieval procedure (citrate buffer, pH 6.0) was performed for 10 min.

A pretreatment microwave heating in 10 m M citrate buffer, pH 6.0, was performed before incubation with the primary antibody.

After rinsing with PB, pH 7.5, blocking of unreacted carboxylic groups with 0.1 M solution of AEE in 0.1 M bicarbonate buffer, pH 7.0, was performed.

After sectioning at 7 μm, dewaxing and rehydration, an antigen retrieval step of 90°C for 60 min in 10 mM sodium citrate buffer pH 6.0 was performed.

22 An antigen retrieval step using citrate buffer (pH 6.0) was performed and then sections were incubated with rabbit polyclonal anti-AnxA1 (1:1000) (Zymed Laboratories, Cambridge, UK) or anti-FPR2 (1:4000).

Briefly, heat induced epitope retrieval with citrate buffer (pH 6.0) was performed, followed by incubation in 3.5 N HCl for 15 min at room temperature.