Sentence examples for buffer of the protein from inspiring English sources

The buffer of the protein solution was exchanged to 1 ml of PBS buffer using a centrifugal device (Amicon Ultra-15, Millipore, Billerica, MA, USA).

For this, the Tris HCl buffer of the protein sample was exchanged to NH4CO2H (5 mM, pH 7.0) using a Nanosep centrifugal device (PALL Life Sciences).

Finally the buffer of the protein was changed to 1 mM potassium phosphate buffer, pH 7, by two series of dialysis and concentrated to 850 µM.

Prior to crystallization, the buffer of the protein sample was exchanged by gel filtration to 20 mM Bis-Tris (pH 6.0), 150 mM NaCl and concentrated to 10 mg/ml.

Lyophilized peptides were resuspended in the final buffer of the proteins (10 mM Tris-HCl pH 7.4, 100 mM NaCl).

The buffer of the eluted protein fractions was exchanged for protein storage buffer (50 mM KPO4 pH 7.8, 300 mM KCl, 1 mM EGTA, 0.5 mM DTT, 10% glycerol) using a PD10 column according to the manufacturers protocol (GE Healthcare), before flash freezing in liquid nitrogen and storing at −80°C.

The buffer of the purified protein fractions was exchanged into TEVp reaction buffer by using PD-10 desalting columns.

The ammonium sulphate pellet was suspended in appropriate volume of Buffer A and loaded onto 8 ml Mono-Q column (Amersham Life Sciences) equilibrated with 10 column volumes (80 ml) of Buffer A. Following a wash with 3 column volumes of buffer A, the protein of interest was eluted with 10 column volumes of Buffer B (Buffer A+1M NaCl-Linear gradient) and the peak fractions were pooled.

The buffer of the eluted proteins was exchanged (20 mM phosphate buffer, pH 8.0, 150 mM NaCl) by a PD-10 column.

Reformulation from phosphate buffer to succinate buffer increased adherence of the protein to aluminum hydroxide adjuvant from 15 to 91%, with a concomitant increase of nearly threefold in effective antigenicity in a mouse model.

Activities of CO2 aq), NH3 aq), H2S aq) and H2O were calculated using a buffer consisting of the proteins from the METXX organisms listed in Table 1.