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If a buffer method is used, a buffer radius of more than 1 km should be considered.
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20 When a buffer method was used, there was a wide range of buffer distances, ranging from 100 m 21 to 5 km, 22 with two studies examining multiple distances for what is considered walkable (eg, 500 m and 1 km) to needing transportation (eg, 2 and 5 km).
Some modifications in assay conditions (Buffered Method) were used to control potential variables across the experiment (including effects of brain pH and other brain components on the reaction).
For p-AKT (ser473; Cell Signaling, 1/50 dilution) and p-ERK1/2 (Cell Signaling, 1 100 dilution), citrate buffer and microwave antigen retrieval method was used.
The double-capsule method was used to buffer oxygen fugacity with a solid powder Ni-NiO (10 1, wt).
In the first WB experiments, wet-ice cooled PBS and a lysis buffer containing phosphatase and protease inhibitors (see methods) was used to extract the protein.
Voltammetric methods were used to study the electrochemical behaviour of hydrazine on MBCPE/Fe3O4NPs/DPB in phosphate buffer solution (pH = 7.0).
What methods were used?
Three isolation methods were used.
Propose a method in which the z-buffer approach is used, but memory is allocated for only part of the screen.
Therefore, for simplicity of the method no buffer was used throughout the study.
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