Sentence examples for buffer membrane from inspiring English sources

The tether extraction force did not increase during tether formation, which suggests existence of a membrane reservoir intended to buffer membrane tension fluctuations.

After two washing steps in PEB buffer, membrane-bound ribosomes were released by incubating pellets in PEB buffer supplemented with 1% (v/v) Triton X-100 for 30 min on ice.

After blocking with 5% (w/v) non-fat dried milk in 10 mM Tris HCl, pH 7.4, 150 mM NaCl, and 0.05% Tween-20 (TTBS) buffer, membranes were incubated with the appropriate primary antibodies, i.e. rabbit polyclonal anti-caspase-3 (Cell Signaling Technology, Hertfordshire, UK) or mouse monoclonal anti-actin (Neomarkers, Lab Vision Corporation, Fremont, CA, USA).

After 3 washes with cold wash buffer, membrane-associated radioactivity was determined by autoradiography and quantification was done by measuring membrane counts in a scintillation counter.

After washing with TBST buffer, membranes were incubated with appropriate secondary antibodies coupled to peroxidase.

After three washes in PBST buffer, membranes were incubated with the corresponding secondary antibody.

Following incubation with 5%milk/0.1%% Tween 20 (Thermo Fisher)/TBS blocking buffer, membranes were subjected to immunoblotting.

Following washing with PBST buffer, membranes were incubated with secondary antibody at appropriate dilution in 5 mL 10% blocking buffer.

After washing with TBST buffer, membranes were incubated overnight at 4 °C with relevant antibodies to ERα and β-actin separately in blocking buffer.

After washing 3 times with TBS/T buffer, membranes were incubated with a horseradish peroxidase-conjugated secondary antibodies (Boster, Wuhan, China) for 1 h.

After washing with TBS-Tween buffer, membranes were incubated with donkey anti-rabbit peroxidase-conjugated IgG 1 20,000 dilution, (Amersham Bioscienses, Piscataway, NJ), for 1 h at room temperature.