Sentence examples for buffer lacking the protease from inspiring English sources

Beads were washed three times with 10 volumes of lysis buffer lacking the protease inhibitor cocktail.

High speed supernatant was recirculated over a 5 ml HiTrap chelating column (GE Healthcare, Cat# 17-0409-03) that was charged with 100 mM CoCl2 (Sigma, Cat# 255599), washed with water, and then equilibrated with lysis buffer lacking protease inhibitors and DNase.

Proteins were eluted in 10 CV of lectin binding buffer lacking detergent and supplemented with 200 mM l-Fuc and protease inhibitors.

Then, cells were lysed in the NETN buffer with the protease inhibitor and were incubated with the Sepharose immobilized with indicated proteins at 4 °C for 8 h.

For immunoblotting, cell lysates extracted with lysis buffer (Fisher) containing the protease inhibitor cocktail (Roche) were loaded into 4 12% Bis-Tris precast gels (Bio-Rad) for electrophoresis.

First, cathepsin B lacks the ERFNIN motif, so that the protease lacks most of the α2 helix found in cathepsin L sub-family proteases.

However, these compounds lacked the selectivity of other serine proteases in the coagulation cascade, especially thrombin.

"A strange and buffer cry", the words that appear on the book, lack the same impact.

Whole cell lysates were harvested using RIPA Lysis and Extraction Buffer (Invitrogen) supplemented with the protease inhibitor cocktail (Sigma).

Samples were homogenized using an Ultraturax homogenizer in lysis buffer containing the provided protease inhibitors.

Cell lysates were prepared with RIPA buffer containing the complete protease mix (Roche).