Sentence examples for buffer kit from inspiring English sources

Ca2+ titration was done with premixed Ca2+ buffers (Calcium Calibration Buffer Kit #2 and #3, Invitrogen).

Intracellular staining for FoxP3, CTLA-4 and cytokines was performed using the eBioscience fixation/permeabilization buffer kit following manufacturer's instructions.

Bacterial extracts were processed for protein purification using a His-Bind Resin column and buffer kit (Novagen).

Cross-linked cell lysate was prepared using a RIPA buffer kit (Santa Cruz) as per the manufacturer's instructions.

Ca2+ calibration of Fura-2 fluorescence was performed in vitro using the Ca2+ calibration Buffer Kit #2 (Invitrogen-Molecular Probes, Eugene, OR).

The fusion protein was purified by affinity chromatography with the HisBind resin and buffer kit, in accordance with the manufacturer's instructions (Novagen/Merck Chemicals, Nottingham, UK).

For the 5' end regions with high GC contents at the wx gene, LA Taq™ with a GC Buffer kit (Takara) was used.

Calibration, enabling linking fluorescence intensity with intracellular concentration, was performed using the calibration buffer kit, exposing calcium probe-loaded cells to buffers which free Ca2+ concentration was set between 0 and 39 µM with appropriate EDTA concentrations.

Cells were then fixed and permeabilized with the Cytofix/Cytoperm buffer kit (Pharmingen), and stained with APC conjugated anti-IL-2 (Pharmingen), fluorescein-isothiocyanate (FITC -conjugated anti-IFITC -conjugated), phycoerythrin (PE) cyanti-IFN-γ7)-conjugated anti-TNF-α (Pharmingen) and phycoerythrin anti-CD154 (CD40L) (PEarmingen).

Pretreated cells were re-suspended in assay buffer (Kit components) and seeded (1.5x10) in each well.

The ascites or pleural fluid (100 1000 mL) was centrifuged at 1000 rpm at room temperature for 3 minutes; the cell pellets were washed twice with phosphate buffered saline (PBS), and red blood cells were removed using a BD Pharm Lyse™-Lysing Buffer kit (BD Bioscience, NJ), according to the manufacturer's instructions, and washed again two times with PBS.