Sentence examples for buffer is prepared by from inspiring English sources

This buffer is prepared by a mixture of 0.1 M citric acid and 0.2 M sodium monohydrogen phosphate.

This buffer is prepared by boiling PBS containing 1% BSA (pH 7.4) at 100°C until the solution becomes opaque (approximate 14 min) and then chilled on ice.

This buffer is prepared by adding NaN3 to a final concentration of 0.01% and propidium iodide (Molecular Probes, Eugene, OR, http://www.probes.com) to a final concentration of 2μg/ml to PBS/BSA.

One ml of this buffer is prepared by adding 0.1 ml of 10 times polymix, 0.05 ml of 20 times KP and 0.02 ml of 50 mM DTE to 0.83 ml of water.

The phosphate buffer was prepared by taking 2.448 g of potassium dihydrogen phosphate in 1000 mL of water.

TBS buffer was prepared by mixing 150 mM NaCl, 50 mM Tris and 5 mM NaHCO3 followed by stirring over chelex overnight and subsequent filtration.

Tris buffer was prepared by mixing 100 ml 0.1 M tris(hydroxymethyl)aminomethane with 29.4 ml of 0.1 M HCl [13].

Ammonium acetate buffer was prepared by mixing equal volumes of 0.2 M acetic acid (Merck, Darmstadt, Germany) and 0.2 M ammonia (Merck, Darmstadt, Germany), and the pH was adjusted to 5.5 by adding 0.2 M acetic acid or 0.2 M ammonia.

The staining buffer was prepared by diluting an X-gal stock (1g X-gal in 40 ml dimethylformamide) 1∶25 in LacZ wash buffer and adding 5 mM K-ferrocyanide and 5 mM K-ferricyanide.

A stock solution of phosphate buffer was prepared by adding 50 mM disodium hydrogen phosphate aqueous solution to 50 mM sodium dihydrogen phosphate solution until a pH of 6.8 was achieved on a pH meter.

A 0.1 M borate buffer was prepared by dilution of the commercial buffer with DI water.