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Buffer injection was performed as described previously [ 35].
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In each experiment, the electrode was exposed to a flowing 5 s bolus of 1 μM serotonin, followed by 10 s of flowing buffer, and then a subsequent serotonin injection was performed.
Sample injection was performed manually by on off switching of the syringe pump delivering buffer 2. In the diffusion-based separation and the CE separation mode, three-layer flow composed of sample solution, collection buffer (buffer 1), and buffer 2 occurs in the 300 μm square-PDMS channel along the buffer 2 flow channel, as illustrated in Fig. 1.
Injection was performed at 30 μl min−1 for 3 min, followed by 6 min of buffer only.
Injection was performed at 30 μl min−1 for 3 min, followed by 6 min of buffer only, for monitoring of dissociation.
The injection was performed within 3 s.
Each radiotracer injection was performed simultaneously to the injection of fluorescent microspheres.
Injection was performed in a splitless mode at an injection temperature of 280°C.
Sham injection was performed for five animals by injection of sterile PBS.
Intrathymic bone marrow injection was performed as previously described [23].
KA injection was performed as previously described [6].
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