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The very weak immunoreactivity of the fractions obtained while applying glycinate buffer indicated that most of the immunoglobulins were bound to the protein A (Figure 7C).
As well, the high immunoreactivity of fractions obtained while applying the citrate buffer indicated the efficient elution of the immunoreactive immunoglobulins by the citrate buffer (Figure 7D), and most of the bound immunoglobulins were eluted in the fractions No. 3 7.
To examine p38 and ERK1/2 phosphorylation, untreated and treated cells were extracted with the buffer indicated above.
The following primary and secondary antibodies were used at the dilutions (in blocking buffer) indicated: anti-ATAD-3 (rabbit, 1∶200, see below), anti-a-Tubulin (mab4A1, mouse, 1∶40, [50]).
Immunoblotting of crude extracts (CE) prepared by directly boiling agroinfiltrated tissues in Laemmli buffer indicated that HA-CaNDR1a proteins were succesfully expressed in plant cells.
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Lastly, long term stability measurements are shown in buffer indicating the sensor lifetime to be about 10 hrs.
CEPs were released from cell-wall via incubation in calcium-free buffer, indicating the enzyme is liable to self-digestion and ionic misfolding.
Bromination reaction activity of the complexes has been evaluated by the method with phenol red as organic substrate in the presence of H2O2, Br– and phosphate buffer, indicating that they can be considered as potential functional model vanadium-dependent haloperoxidases.
The results in Fig. 2c show that the TRPL signal of perovskite film covered with CdSe/LiF has a faster decay as compared to the film without cathode buffer, indicating a rapid charge injection from MAPbI3 to CdSe.
As shown in Figs. 3 and 4, in 1 M KHCO3, after P4VP loading, the reduction currents dropped for both copper polycrystalline and Cu/GDL like in the case of phosphate buffer indicating HER suppression.
PAniTNC1-modified GCE immersed in 1.0 M H2SO4 at 25 ± 2 °C for 2 s shows a large enhancement in charge in O2-saturated buffer when compared to N2 and normal buffer, indicating that the nitrogen and oxygen buffer interferes with the nanocomposite layer, thereby increasing the charge.
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