Sentence examples for buffer in the sample from inspiring English sources

The final concentration of a borate buffer in the sample solution was 2.5 · 10−4 М.

A detailed discussion on the concentration of separation buffer in the sample is given in Sect.

All RNA samples were then treated with DNase I (New England Biolabs) at 37° for 10 min following inactivation at 75° for 10 min. An additional cleaning step with phenol was usually required in order to remove any DNase or buffers left in the sample.

Then, the beads were washed three times with lysis buffer and boiled in the sample buffer.

After purification, 2 μL of assay buffer (in which the samples were dissolved later) were pipetted onto 16 spots on a NanoQuantPlate (TecanGroup) as blank.

200 µl of 1 mM WT-ACD and 300 µl buffer were placed in the sample and reference cells, respectively.

The peptide fragments formed during digestion were concentrated on an RP trapping column (TC) and salts and other buffer components present in the sample were removed.

These two parameters may be determined by collecting visibility and phase data from the coverslip-buffer interface in the "sample-free" region where B equals zero.

For sample preparation, 100 mg of the single, random stool specimen was extracted in 10 ml extraction buffer and diluted 1 : 10 in the sample washing buffer provided.

Although the exact mode of cell lysis is not clearly understood, it appears to be the combined action of NaOH in the pre-lysis buffer and of 2-mercaptoethanol in the sample buffer that makes cell walls porous enough for proteins to escape into the surrounding buffer.

Immunoprecipitates were collected and washed 3 times with the lysis buffer, resuspended in the SDS sample buffer and heated for 5 minutes at 90°C.