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Measurements were performed using a standard scan rate of 125°/h with dialysis buffer in the reference cell.
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A slightly larger volume (430 μL) of dialysis buffer was placed in the reference compartment.
Association and dissociation curves were double referenced against the buffer reference signal (biosensors coated with ligands and incubated in buffer only), and the reference sensors signal (biosensors without ligands and incubated in AcrIIA6).
A reference cuvette filled with buffer solution was placed in the reference beam.
Reference spectra were recorded under identical conditions with only the reference buffer in the cell.
A few microlitters of concentrated substrate (378 μM thioanisole or 15 mM phenol) in 50 mM sodium citrate buffer (pH 5.0) were added to the CPO solution in the sample cell and an equivalent volume of buffer to the enzyme solution in the reference cell.
A linear temperature ramp from 20 to 100°C was applied at a scan rate of 1 K per min. Prior to all measurements, samples were degassed by continuous stirring in vacuo for 15 min. Obtained sample thermograms were corrected for the calorimeter baseline by subtracting a buffer blank that was scanned in the reference chamber.
The reconstructed frame is now ready for display and is stored in the reference frame buffer, serving as a reference for future temporal prediction.
The decoder first conventionally intra decodes the key frame bit stream and stores the reconstructed frame in the reference frame buffer.
Reactions of NAMI-A, KP1339 and RuEDTA with NO were carried out in the reference phosphate buffer and analysed by absorption and FT-IR spectroscopies and by H-NMR.
Here, the OTR curve followed the same shape as in the reference cultivation with 0.2 M MOPS buffer depicted in Figure 4a.
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