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The mobile phase which gives rapid separation of CTZ, PPA and NMS in good resolution is methanol: 0.1 M phosphate buffer in the ratio (60: 40, v/v).
Briefly, 50 μl reagent (fluorometric substrate: buffer in the ratio of 1 100) was added to each well.
The samples were then immersed in His-GFP solution diluted with 20 mM Tris buffer in the ratio of 1 20.
The fresh working solution was prepared by mixing TPTZ solution, FeCl3 6H2O solution, and acetate buffer in the ratio of 1 : 1 : 10 and it was warmed at 37°C for 25 min before use.
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The total number of surveys issued at each case study site was divided between these four sampling buffers in the ratio 2 1 1 1.
Mobile phase consisted of methanol and 18 mM phosphate buffer (pH 2.8) in the ratio of (62 38 v/v, respectively).
The used mobile phase composed of 0.1 M phosphate buffer and methanol in the ratio of 40 60, v/v at pH 7.0.
Dried residue was reconstituted with 300 μL of reconstitution solution consisting of acetonitrile and 5 mM ammonium acetate buffer (pH 3.6) in the ratio of 60 40, v/v.
A mobile phase consisting of methanol, acetonitrile and 5 mM phosphate buffer (pH 2.5) in the ratio 70.0 2.5 27.5 eluted paclitaxel from a 20 μl injection in 7 8 min when pumped at 0.7 ml/min.
A mobile phase consisting of acetonitrile and 5 mM ammonium acetate buffer (pH 3.6) in the ratio of 90:10, v/v, was delivered at a flow rate of 0.7 mL/min.
Lovastatin and lovastatin-d3 were extracted from human plasma using solid phase extraction, separated on Luna C18 (2)100A (100 × 4.6 mm, 5 μm) column with mobile phase consisting of acetonitrile and 2 mM ammonium acetate buffer (pH 3.6) in the ratio of 90:10, v/v.
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