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Give yourself a twenty minute buffer in the morning- this way you won't be in such a hurry or be as careless as you normally would.
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The sections was washed several times in 0.1 M potassium phosphate buffer, and then put in a 25% (w/v) sucrose in potassium phosphate buffer in 4°C over night.
Most took place in the late morning.
Waking in the early morning.
Nap in the early afternoon.
Feed in the early evening.
Spray in the early evening.
In The Times this morning.
There are several buffers in the body.
The nitrocellulose membrane was blocked with 3% milk powder for 1 hour at room temperature and washed thrice with buffer A (10 mM tris HCl pH 8, 1 mM EDTA pH 8, 0.05% tween 20 and 0.9% NaCl), followed by incubation in 1 : 100 dilution of the rabbit antiserum in the same buffer, at 4°C overnight.
Samples were then deparaffinized, rehydrated, and then antigen retrieval was performed by heating the samples in 10 mM citrate buffer (pH 6) for 1 hour in a steam chamber, then incubating the samples (still in the citrate buffer) at 4°C overnight.
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