Sentence examples for buffer in the dark from inspiring English sources

For unwinding of the DNA, the slides were immersed in 1 × TBE buffer in the dark at 4 °C for 30 min.

In case of the Annexin V-FITC Apoptosis Detection Kit the hyphae on cover slips were washed with CM and stained with PI and Annexin V-FITC diluted in binding buffer in the dark at room temperature for 10 min, and then washed again with CM.

The cells were then washed 3 times and incubated for 60 minutes in ACSF buffer in the dark.

Electrophoresis was carried out in the same buffer in the dark for 30 min at 25 V and 300 mA.

Pistils were softened in 1 M NaOH for 25 35 min at 55°C and stained overnight with 0.1% (w/v) aniline blue in 50 mM KPO4 buffer in the dark.

Proteins were reduced with TCEP (25 mM in denaturing urea buffer), for 15 min at room temperature and alkylated with iodoacetamide (55 mM in denaturing urea buffer), in the dark for 45 min at room temperature.

They were immersed for 1 h in this buffer (4°C, in the dark).

Samples were incubated in 20 μl ELF substrate-buffer solution in the dark for 20 min, then washed 3 × 1 min with wash buffer (PBS with 25 mM EDTA and 5 mM levamisole, pH 8.0) before the samples were mounted.

The slides were rinsed in a Tris-buffered saline (TBS) series and visualized with a 10-min incubation of liquid 3,3′-diaminobenzidine in buffered substrate in the dark.

After washing twice in permeabilization buffer, Fc-blockage was performed in permeabilization buffer at +4 °C in the dark for 10 min.

Following incubation of the transfected and non-transfected cells with 50 μl FcR blocking reagent (Miltenyl Biotec) for 15 min at 4°C and washing in 10 ml staining buffer, the cells were fixed in 500 μl IC Fixation buffer (eBioscience, Frankfurt, Germany) in the dark for 20 min.